ABSTRACT
Abstract This study aimed to evaluate the contribution of oral and maxillofacial pathology laboratories (OMPLs) in Brazilian public universities to the diagnosis of lip, oral cavity, and oropharyngeal squamous cell carcinoma (SCC). A cross-sectional study was performed using biopsy records from a consortium of sixteen public OMPLs from all regions of Brazil (North, Northeast, Central-West, Southeast, and South). Clinical and demographic data of patients diagnosed with lip, oral cavity, and oropharyngeal SCC between 2010 and 2019 were collected from the patients' histopathological records. Of the 120,010 oral and maxillofacial biopsies (2010-2019), 6.9% (8,321 cases) were diagnosed as lip (0.8%, 951 cases), oral cavity (4.9%, 5,971 cases), and oropharyngeal (1.2%, 1,399 cases) SCCs. Most cases were from Brazil's Southeast (64.5%), where six of the OMPLs analyzed are located. The predominant profile of patients with lip and oral cavity SCC was Caucasian men, with a mean age over 60 years, low schooling level, and a previous history of heavy tobacco consumption. In the oropharyngeal group, the majority were non-Caucasian men, with a mean age under 60 years, had a low education level, and were former/current tobacco and alcohol users. According to data from the Brazilian National Cancer Institute, approximately 9.9% of the total lip, oral cavity, and oropharyngeal SCCs reported over the last decade in Brazil may have been diagnosed at the OMPLs included in the current study. Therefore, this data confirms the contribution of public OMPLs with respect to the important diagnostic support they provide to the oral healthcare services extended by the Brazilian Public Health System.
ABSTRACT
The radiographic features of an intraosseous lesion are usually associated with the biological behavior of the tumor. In view of the fact that the growth and behavior of keratocystic odontogenic tumors (KCOT) is mainly associated with the proliferation of the cystic epithelium, the objective of the present study was to evaluate the relationship between cell proliferation markers and radiographic features of this tumor. Thirty-seven radiographs of KCOT obtained from 30 patients were scanned and evaluated on a monitor. Sections were submitted to immunohistochemistry for Ki-67, p63, and p53 proteins on an EnVision system. Thirty-one KCOTs were observed in the posterior of the mandible, and the unilocular aspect was predominant (n= 26). Nineteen KCOTs distorted the mandibular canal and 11 displaced teeth. Satellite cysts were associated with a multilocular aspect (P= 0.016). p53 was in KCOTS with diffuse margins (p=0.049), p63 with NBCCS (p=0.049) KOT and higher KI-67 positive cells was observed in KCOTs presenting distortion of the mandibular canal (p=0.042). The distribution of Ki-67, p63, and p53 positive cells was similar between KCOTs with uni- and multilocular aspects. The results of the present study suggest that cell proliferation in KCOT contributes to the radiographic features of this tumor.
Las características radiográficas de una lesión intraósea se asocian generalmente con el comportamiento biológico del tumor. Debido a esto, el crecimiento y comportamiento de los tumores odontogénicos queratoquísticos se asocian principalmente con la proliferación del epitelio quístico. El objetivo del estudio fue evaluar la relación entre los marcadores de proliferación celular y las características radiológicas de este tumor. Se escanearon y evaluaron 37 radiografías de tumores odontogénicos queratoquísticos obtenidos de 30 pacientes y las secciones de sus biopsias fueron sometidas a evaluación inmunohistoquímica para las proteíneas Ki-67, p63 y p53 en un sistema Envision. Se observaron 31 tumores odontogénicos queratoquísticos en el área posterior de la mandíbula, con predominio del aspecto unilocular (n= 26). Diecinueve tumores odontogénicos queratoquísticos distorsionaron el canal mandibular y se observaron 11 dientes desplazados. Los quistes satélites se asociaron con el aspecto multilocular (P= 0,016). La distribución de células positivas para Ki-67, p63 y p53 fue similar entre tumores odontogénicos queratoquísticos con aspectos uniformes y multiloculares, y no estaban relacionadod con la distorsión del canal mandibular (P>0,05) o con el desplazamiento del diente (P>0,05). Los resultados del presente estudio sugieren que la proliferación celular en tumores odontogénicos queratoquísticos contribuye a las características radiográficas de este tumor.